You need to remove the dome, just in case it has a pipe up into it from the regulator tube.
if it has a pipe ,remove it.
Now, smokebox end .
When you removed all that pipework , you should have seen a piece of plain pipe with an O-ring seal sticking out
into the end with all the pipes on.
Pray you did , because otherwise it will be impossible to get out without breaking something.
Back to the large flanged end on the boiler backhead.
You say you have removed all the screws, well done.
Now you have to be brutal and somehow or other grip, pull and twist that flange out of the boiler.
All of the regulator tube has to come out.
There is no other way.